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Prototype design of the LumiPore™ photoporation platform for gentler cell transfections.
The cell transfection process by photoporation for R&D applications.
The cell transfection process by photoporation for cell therapy development.
Watch our video to see how easy it is.
The LumiPoreTM technology is very gentle to cells, ensuring minimal impact on cell viability, cell proliferation, homeostasis and functionality. This has for instance been demonstrated on hard-to-transfect human T cells, which proliferate almost immediately and maintain their full cell-killing potential after transfection with the LumiPoreTM system.
Key:
NTC – non-treated cells
PEN – photothermal electrospun nanofibers (photoporation)
EP – electroporation
Proliferation of activated T cells after transfection by photoporation happens equally as fast as non-treated T cells, while electroporated T cells show delayed proliferation. Reference: Xiong et al. Nat Nanotechnol (2021)
The LumiPoreTM platform is a gentler technology than the current state-of-the-art electroporation technologies and therefore improves the transfection yield, resulting in a higher number of transfected and viable cells. For instance, photoporation leads to 2.5 times more living mRNA-transfected stimulated human T cells as compared to the current gold standard electroporation method. Reference: Harizaj et al. Adv Funct Mater (2021)
Reference: Harizaj et al. Adv Funct Mater (2021)
The LumiPoreTM technology offers high throughput with limited hands-on time, making it suitable for a large variety of applications including screening assays. After adding the proprietary nanosensitizers and effector molecules to the cells, the recipient is placed in the device and the transfection can be started with a simple click of a button. An entire multi-well plate is transfected in less than ten minutes.
LumiPoreTM offers the unique possibility to transfect specific subpopulations of cells. For instance, cells can be transfected according to user-defined spatial patterns. This opens up unique experimental solutions for tissue engineering applications or studying bystander effects.
Additionally, the LumiPoreTM platform can optionally integrate transmission and fluorescence imaging of cells. This allows for the delivery of payloads to specific cells which are either selected manually or automatically by making use of automated in-line image processing. Transfection can be done with single cell resolution.
Illustrated here is the selective delivery of two different fluorescent labels to adjacent neurons, facilitating interaction studies between these cells.
Photoporation can deliver different fluorescent labels to adjacent cells with precision targeting.
Trince Bio provides all consumables required for operating the LumiPore™ photoporation platform.
Trince Bio is looking for early access partners to test and validate the LumiPoreTM platform and provide feedback from real-world applications.
Get involved early and help us to streamline our development process and provide the best solution for your needs!
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