Transfection is frequently performed in stem cells and cancer cell lines to explore pluripotency, unravel mechanisms of early embryonic development, and facilitate in vivo tracking of specific stem cells and their progeny.
Additionally, it serves as a powerful tool for reprogramming somatic cells and engineering stem cells, whether through genome editing techniques like CRISPR/Cas9 or by modulating gene expression at the RNA level using RNA interference (RNAi).
An attractive solution is Trince’s LumiPore® transfection platform, which combines efficiency with gentleness. It combines laser exposure with photothermal nanoparticles to transiently permeabilize the cellular plasma membrane, allowing external cargo molecules to penetrate the cells.
